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In vitro analysis of cytotoxicity, cellular uptake, and Hsp70 promoter activation. (a) Relative cell viability of <t>HSF</t> <t>and</t> <t>MDA‐MB‐231</t> cells after different concentrations of IONP@H 1 THs treatment (n = 3). (b) Quantitative analysis of cellular uptake of IONP@H 1 THs (n = 3). MDA‐MB‐231(‐), the cells treated with a nanoplatform without Aptamer DNA. (c) TEM images of HSF and MDA‐MB‐231 cells after IONP@H 1 THs treatment. (d‐e) IFA staining and quantitative analysis of Hsp70 expression in MDA‐MB‐231 cells after different treatments (n = 3). Heat, the cells were directly heated at 43°C for 30 min, respectively. (f‐g) Western blot and quantitative analysis of Hsp70 expression in MDA‐MB‐231 cells after different treatments (n = 3), respectively. (h) Quantitative real‐time polymerase chain reaction analysis of Hsp70 mRNA expression in MDA‐MB‐231 cells after different treatments (n = 3). (i) AMF‐mediated specific activation of Hsp70 promoter using a reporter gene of mCherry (n = 3). Mean ± SD. * p <0.05; *** p <0.001; ns, not significant; one‐way ANOVA followed by Tukey post hoc test (b, e, g, h).
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In vitro analysis of cytotoxicity, cellular uptake, and Hsp70 promoter activation. (a) Relative cell viability of <t>HSF</t> <t>and</t> <t>MDA‐MB‐231</t> cells after different concentrations of IONP@H 1 THs treatment (n = 3). (b) Quantitative analysis of cellular uptake of IONP@H 1 THs (n = 3). MDA‐MB‐231(‐), the cells treated with a nanoplatform without Aptamer DNA. (c) TEM images of HSF and MDA‐MB‐231 cells after IONP@H 1 THs treatment. (d‐e) IFA staining and quantitative analysis of Hsp70 expression in MDA‐MB‐231 cells after different treatments (n = 3). Heat, the cells were directly heated at 43°C for 30 min, respectively. (f‐g) Western blot and quantitative analysis of Hsp70 expression in MDA‐MB‐231 cells after different treatments (n = 3), respectively. (h) Quantitative real‐time polymerase chain reaction analysis of Hsp70 mRNA expression in MDA‐MB‐231 cells after different treatments (n = 3). (i) AMF‐mediated specific activation of Hsp70 promoter using a reporter gene of mCherry (n = 3). Mean ± SD. * p <0.05; *** p <0.001; ns, not significant; one‐way ANOVA followed by Tukey post hoc test (b, e, g, h).
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In vitro analysis of cytotoxicity, cellular uptake, and Hsp70 promoter activation. (a) Relative cell viability of <t>HSF</t> <t>and</t> <t>MDA‐MB‐231</t> cells after different concentrations of IONP@H 1 THs treatment (n = 3). (b) Quantitative analysis of cellular uptake of IONP@H 1 THs (n = 3). MDA‐MB‐231(‐), the cells treated with a nanoplatform without Aptamer DNA. (c) TEM images of HSF and MDA‐MB‐231 cells after IONP@H 1 THs treatment. (d‐e) IFA staining and quantitative analysis of Hsp70 expression in MDA‐MB‐231 cells after different treatments (n = 3). Heat, the cells were directly heated at 43°C for 30 min, respectively. (f‐g) Western blot and quantitative analysis of Hsp70 expression in MDA‐MB‐231 cells after different treatments (n = 3), respectively. (h) Quantitative real‐time polymerase chain reaction analysis of Hsp70 mRNA expression in MDA‐MB‐231 cells after different treatments (n = 3). (i) AMF‐mediated specific activation of Hsp70 promoter using a reporter gene of mCherry (n = 3). Mean ± SD. * p <0.05; *** p <0.001; ns, not significant; one‐way ANOVA followed by Tukey post hoc test (b, e, g, h).
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In vitro analysis of cytotoxicity, cellular uptake, and Hsp70 promoter activation. (a) Relative cell viability of <t>HSF</t> <t>and</t> <t>MDA‐MB‐231</t> cells after different concentrations of IONP@H 1 THs treatment (n = 3). (b) Quantitative analysis of cellular uptake of IONP@H 1 THs (n = 3). MDA‐MB‐231(‐), the cells treated with a nanoplatform without Aptamer DNA. (c) TEM images of HSF and MDA‐MB‐231 cells after IONP@H 1 THs treatment. (d‐e) IFA staining and quantitative analysis of Hsp70 expression in MDA‐MB‐231 cells after different treatments (n = 3). Heat, the cells were directly heated at 43°C for 30 min, respectively. (f‐g) Western blot and quantitative analysis of Hsp70 expression in MDA‐MB‐231 cells after different treatments (n = 3), respectively. (h) Quantitative real‐time polymerase chain reaction analysis of Hsp70 mRNA expression in MDA‐MB‐231 cells after different treatments (n = 3). (i) AMF‐mediated specific activation of Hsp70 promoter using a reporter gene of mCherry (n = 3). Mean ± SD. * p <0.05; *** p <0.001; ns, not significant; one‐way ANOVA followed by Tukey post hoc test (b, e, g, h).
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In vitro analysis of cytotoxicity, cellular uptake, and Hsp70 promoter activation. (a) Relative cell viability of HSF and MDA‐MB‐231 cells after different concentrations of IONP@H 1 THs treatment (n = 3). (b) Quantitative analysis of cellular uptake of IONP@H 1 THs (n = 3). MDA‐MB‐231(‐), the cells treated with a nanoplatform without Aptamer DNA. (c) TEM images of HSF and MDA‐MB‐231 cells after IONP@H 1 THs treatment. (d‐e) IFA staining and quantitative analysis of Hsp70 expression in MDA‐MB‐231 cells after different treatments (n = 3). Heat, the cells were directly heated at 43°C for 30 min, respectively. (f‐g) Western blot and quantitative analysis of Hsp70 expression in MDA‐MB‐231 cells after different treatments (n = 3), respectively. (h) Quantitative real‐time polymerase chain reaction analysis of Hsp70 mRNA expression in MDA‐MB‐231 cells after different treatments (n = 3). (i) AMF‐mediated specific activation of Hsp70 promoter using a reporter gene of mCherry (n = 3). Mean ± SD. * p <0.05; *** p <0.001; ns, not significant; one‐way ANOVA followed by Tukey post hoc test (b, e, g, h).

Journal: Advanced Science

Article Title: Intelligent Thermo‐Self‐Limited Magnetothermia with Heat‐Triggered TERT Silencing for Precision Synergetic Cancer Therapy

doi: 10.1002/advs.202519168

Figure Lengend Snippet: In vitro analysis of cytotoxicity, cellular uptake, and Hsp70 promoter activation. (a) Relative cell viability of HSF and MDA‐MB‐231 cells after different concentrations of IONP@H 1 THs treatment (n = 3). (b) Quantitative analysis of cellular uptake of IONP@H 1 THs (n = 3). MDA‐MB‐231(‐), the cells treated with a nanoplatform without Aptamer DNA. (c) TEM images of HSF and MDA‐MB‐231 cells after IONP@H 1 THs treatment. (d‐e) IFA staining and quantitative analysis of Hsp70 expression in MDA‐MB‐231 cells after different treatments (n = 3). Heat, the cells were directly heated at 43°C for 30 min, respectively. (f‐g) Western blot and quantitative analysis of Hsp70 expression in MDA‐MB‐231 cells after different treatments (n = 3), respectively. (h) Quantitative real‐time polymerase chain reaction analysis of Hsp70 mRNA expression in MDA‐MB‐231 cells after different treatments (n = 3). (i) AMF‐mediated specific activation of Hsp70 promoter using a reporter gene of mCherry (n = 3). Mean ± SD. * p <0.05; *** p <0.001; ns, not significant; one‐way ANOVA followed by Tukey post hoc test (b, e, g, h).

Article Snippet: MDA‐MB‐231 and HSF cells were purchased from Procell Life Science & Technology Co., Ltd (Wuhan, China).

Techniques: In Vitro, Activation Assay, Staining, Expressing, Western Blot, Real-time Polymerase Chain Reaction